ASTM_E_2645_-_08.pdf

Designation:E264508Standard Specification forFeltiella acarisuga(Vallot)(Diptera:Cecidomyiidae)1This standard is issued under the fixed designation E2645;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification covers information on and the testmethod for determining purity,sex ratio and number of adultsreleased from commercial containers of the predatory midgeFeltiella acarisuga(Vallot)(Diptera:Cecidomyidae).1.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.2.Referenced Documents2.1 ASTM Standards:2E2200 Specification for Information Included with Packag-ing of Multi-Cellular Biological Control Organisms(Withdrawn 2010)33.Terminology3.1 life stage when shippedpupae in cocoons.3.2 name of productFeltiella acarisuga(Vallot)3.3 carrierfolded paper or bean leaves3.4 preferred host preyspidermites4.Classification4.1 PhylumArthropoda.4.2 ClassInsecta.4.3 OrderDiptera.4.4 FamilyCecidomyiidae.4.5 GenusFeltiella.4.6 Speciesacarisuga.5.Summary of Test Method(Determining the Purity,SexRatio,and Number of Adults Released fromContainers of F.acarisuga)5.1 This test method describes a method of counting thenumber of F.acarisuga adult midges released from pupae incocoons packaged on paper or leaf,determining purity and sexratio of adults.This test method is used to test a standardcommercial package of 250 midges sold as pupae in cocoonson folded paper or bean leaves.Adult midges are allowed toemerge over a ten day period,are counted and sex ratiodetermined.5.2 The number of F.acarisuga midges released from pupaein cocoons will be determined by examining a minimum ofthree containers and determining the mean value.Any liveparasitoid or arthropod product contaminants will be recorded.6.Significance and Use6.1 The efficacy of spidermite control by F.acarisugadepends on accurate release numbers of midges,presence ofmale and female adults,and absence of live product contami-nants,especially parasitoids of F.acarisuga.(Accurate pack-aging and maintenance of purity and viability of F.acarisugashipments is,therefore,essential for the effective managementof the target pest.)This test method is intended for use byproducers and users of the specified biological control agent.Itis complementary to the quality guidelines for Feltiella acar-isuga that were developed by the International Organization ofBiological Control and published in Lenteren,J.C.van(ed.),2003.47.Materials7.1 Dissecting microscope or headband magnifier(7 to10).7.2 Clear polyethylene storage bag,2 L volume.7.3 Yellow sticky cards,50 cm2.7.4 Twist-ties.7.5 Fine pointed paint brush.1This specification is under the jurisdiction of ASTM Committee E35 onPesticides and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.30 on Natural Multi-Cellular(Metazoan)Biological ControlOrganisms.Current edition approved Oct.1,2008.Published October 2008.DOI:10.1520/E2645-08.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.4van Lenteren,et al.,“Guidelines for Quality Control of Commercially ProducedNatural Enemies,”Quality Control and Production for Biological Control AgentsTheory and Testing Procedures,J.C.van Lenteren,Ed.,CABI Publishing,2003,pp.278279.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 8.Test Unit8.1 A single container with F.acarisuga is considered a testunit.This test is designed for standard shipments of 250midges.In large shipments,a minimum of three containersshould be chosen at random for testing.9.Pre-Test and Test Conditions9.1Store containers with F.acarisuga at 5C,relativehumidity 60 to 90%,for a maximum of 24 h prior tocommencing test.During tests,use temperature of 22 to 25Cand light regime of 16 L:8 D(out of direct sunlight).10.Sampling10.1 Specify the number of adults that are expected toemerge from each test unit as indicated on the package beforecommencing test.11.Sample Preparation and Treatment11.1 Total adult emergence is checked over a ten day period.Take a 2 Lclear polyethylene bag,prop it open,and adhere oneyellow sticky card to the inside top half of the bag,leavingsufficient space at the top for it to fasten.Draw the open endthrough the air to get as much air into the bag as possible(butdo not use your breath to blow it up),then pinch the top of thebag shut and tie a twist-tie far enough down the bag to force theair to inflate the lower two-thirds of the bag.Thi