Designation:E2144−11AnAmericanNationalStandardStandardPracticeforPersonalSamplingandAnalysisofEndotoxininMetalworkingFluidAerosolsinWorkplaceAtmospheres1ThisstandardisissuedunderthefixeddesignationE2144;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispracticecoversquantitativemethodsfortheper-sonalsamplinganddeterminationofbacterialendotoxincon-centrationsinpoly-dispersemetalworkingfluidaerosolsinworkplaceatmospheres.Usersshouldhavefundamentalknowledgeofmicrobiologicaltechniquesandendotoxintest-ing.1.2Usersofthispracticemayobtainpersonalorareaexposuredataofendotoxininmetalworkingfluidaerosols,eitheronashort-termorfull-shiftbasisinworkplaceatmo-spheres.1.3Thispracticegivesanestimateoftheendotoxincon-centrationofthesampledatmosphere.1.4Thispracticeseekstominimizeinterlaboratoryvaria-tionbutdoesnotensureuniformityofresults.1.5Itisanticipatedthatthispracticewillfacilitateinterlaboratorycomparisonsofairborneendotoxindatafrommet-alworkingfluidatmospheres,particularlymetalremovalfluidatmospheres,byprovidingabasisforendotoxinsampling,extraction,andanalyticalmethods.1.6In1997,theOccupationalSafetyandHealthAdminis-tration(OSHA)empanelledaStandardsAdvisoryCommitteetomakerecommendationstotheAdministrationregardingmeasuresthattheAdministrationcouldtaketoimprovethehealthofworkersexposedtometalworkingfluids.AreporttotheAssistantSecretaryofLaborforOSHAwassubmittedinJuly,1999.SubcommitteeE34.50believesthattheusercom-munitywouldbenefitsignificantlyifastandardmethodwasdevelopedtogivethecommunityguidanceonamethodologyforthesamplingandanalysisofpersonalairborneendotoxinexposureassessmentsinfacilitiesusingwater-misciblemetalremovalfluids,basedontheLALassayorotherendotoxindetectiontechnologiesastheybecomeavailable.1.7Thispracticedoesnotattempttosetorimplylimitsforpe...
