ASTM_E_2106_-_00_2011.pdf

Designation:E210600(Reapproved 2011)Standard Practice forGeneral Techniques of Liquid Chromatography-Infrared(LC/IR)and Size Exclusion Chromatography-Infrared(SEC/IR)Analyses1This standard is issued under the fixed designation E2106;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice covers techniques that are of general use inqualitatively analyzing multicomponent samples by using acombination of liquid chromatography(LC)or size exclusionchromatography(SEC)with infrared(IR)spectrometric tech-niques.The sample mixture is separated into fractions by thechromatographic separation.These fractions are subsequentlyanalyzed by an IR spectroscopic method.1.2 Three different types of LC/IR techniques have beenused to analyze samples(1,2).2These consist of eluenttrapping(see Practices E334),flowcell and direct deposition.These are presented in the order that they were first used.1.3 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.4 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:3E131 Terminology Relating to Molecular SpectroscopyE168 Practices for General Techniques of Infrared Quanti-tative AnalysisE334 Practice for General Techniques of Infrared Micro-analysisE1421 Practice for Describing and Measuring Performanceof Fourier Transform Mid-Infrared(FT-MIR)Spectrom-eters:Level Zero and Level One Tests3.Terminology3.1 DefinitionsFor definitions of terms and symbols,referto Terminology E131.3.2 Definitions of Terms Specific to This Standard:3.2.1 hit quality index(HQI),nthe comparison of infraredspectroscopic data against a database of reference spectra ofknown compounds is often employed to assist in the determi-nation of the evolved gas chemical identity.Search algorithmsgenerate a listing of reference compounds from the databasethat are spectroscopically similar to the evolved gas spectrum.These reference compounds are ranked with regard to ameasurement of the comparative fit of the reference spectraldata to that of the spectrum of the evolved gas.This ranking isreferred to as the hit quality index(HQI).4.Significance and Use4.1 This practice provides general guidelines for the prac-tice of liquid chromatography or size exclusion chromatogra-phy coupled with infrared spectrometric detection and analysis(LC/IR,SEC/IR).This practice assumes that the chromatogra-phy involved is adequate to resolve a sample into discretefractions.It is not the intention of this practice to instruct theuser on how to perform liquid or size exclusion chromatogra-phy(LC or SEC).5.General LC/IR Techniques5.1 Three different LC/IR techniques have been used toanalyze samples.These consist of eluent trapping,flowcell anddirect deposition.These are presented in the order that theywere first developed.Infrared detection for any of thesetechniques can be provided by IR monochromators,IR filterspectrometers and Fourier transform infrared spectrometers(FT-IR).These detectors yield either single absorption band ortotal infrared spectrum detection modes.Detection mode isdependent upon the type of IR detector employed and theacquisition time required by the LC or SEC experiment.1This practice is under the jurisdiction of ASTM Committee E13 on MolecularSpectroscopy and Separation Science and is the direct responsibility of Subcom-mittee E13.03 on Infrared and Near Infrared Spectroscopy.Current edition approved Nov.1,2011.Published December 2011.Originallyapproved in 2000.Last previous edition approved in 2006 as E2106 00(2006).DOI:10.1520/E2106-00R11.2The boldface numbers in parentheses refer to the list of references at the end ofthis standard.3For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 5.2 EluentTrappingTechniquesEluenttrappingtechniques,such as stopped flow and fraction collection,arethe simple means for obtaining LC/IR data.In thesetechniques,the eluting sample is collected from the chromato-graph in discrete aliquots.These aliquots are then analyzedwith the appropriate sampling accessory in an infrared spec-trometer.In utilizing such techniques,it is essential that asuitable LC detector,such as refractive index or UV/VIS,beemployed to allow definition of