ASTM_E_2149_-_13a.pdf

Designation:E214913aStandard Test Method forDetermining the Antimicrobial Activity of AntimicrobialAgents Under Dynamic Contact Conditions1This standard is issued under the fixed designation E2149;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This test method is designed to evaluate the antimicro-bial activity of non-leaching,antimicrobial-treated specimensunder dynamic contact conditions.This dynamic shake flasktest was developed for routine quality control and screeningtests in order to overcome difficulties in using classicalantimicrobial test methods to evaluate substrate-bound antimi-crobials.These difficulties include ensuring contact of inocu-lum to treated surface(as in AATCC 100),flexibility ofretrieval at different contact times,use of inappropriatelyapplied static conditions(as in AATCC 147),sensitivity,andreproducibility.1.2 This test method allows for the ability to evaluate manydifferent types of treated substrates and a wide range ofmicroorganisms.Treated substrates used in this test methodcan be subjected to a wide variety of physical/chemical stressesor manipulations and allows for the versatility of testing theeffect of contamination due to such things as hard water,proteins,blood,serum,various chemicals,and other contami-nants.1.3 Surface antimicrobial activity is determined by compar-ing results from the test sample to controls run simultaneously.1.4 The presence of an antimicrobial that requires neutral-ization is determined by the post-test.1.5 Proper neutralization of all antimicrobials must beconfirmed using Test Methods E1054.1.6 This test method should be performed only by thosetrained in microbiological techniques.1.7 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.8 This standard may involve hazardous materials,operations,and equipment.This standard does not purport toaddress all of the safety concerns,if any,associated with itsuse.It is the responsibility of the user of this standard toestablish appropriate safety and health practices and deter-mine the applicability of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2E1054 Test Methods for Evaluation of Inactivators of Anti-microbial Agents2.2 AATCC Documents:3AATCC 147 Antibacterial Activity Assessment of TextileMaterials:Parallel Streak MethodAATCC 100 Antibacterial Finishes on Fabrics3.Summary of Test Method3.1 The antimicrobial activity of a substrate-bound,non-leaching antimicrobial agent is dependent upon direct contactof microbes with the active chemical agent.This test deter-mines the antimicrobial activity of a treated specimen byshaking samples of surface-bound materials in a concentratedbacterial suspension for a one hour contact time.The suspen-sion is serially diluted both before and after contact andcultured.The number of viable organisms from the suspensionis determined and the percent reduction(or log10reduction)iscalculated by comparing retrievals from appropriate controls.4.Significance and Use4.1 Chemically bonded,antimicrobial agents are not free todiffuse into their environment under normal conditions of use.This test method ensures good contact between the bacteria andthe treated fiber,fabric,or other substrate,by constant agitationof the test specimen in a challenge suspension during the testperiod.1This test method is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and is the directresponsibility of Subcommittee E35.15 on Antimicrobial Agents.Current edition approved Oct.1,2013.Published December 2013.Originallyapproved in 2001.Last previous edition approved in 2013 as E2149 13.DOI:10.1520/E2149-13A.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3Available from American Association of Textile Chemists and Colorists(AATCC),P.O.Box 12215,Research Triangle Park,NC 27709,http:/www.aatcc.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 4.2 The metabolic state of the challenge species can directlyaffect measurements of the effectiveness of particular antimi-crobial agents or concentrations of agents.The susceptibility ofthe species to particular biocides could be altered depending onits life stage(cycle).One-hour contact time in a buffer solutionallows for metabolic stasis in the population.This test methodstandardizes both the growth conditions of the challengespecies and substrate contact times to reduce the variabilityassociated with growth phase of th