Designation:E1924–97StandardGuideforConductingToxicityTestswithBioluminescentDinoflagellates1,2ThisstandardisissuedunderthefixeddesignationE1924;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidecoverstwodistinctprocedures,basedonsimilarprinciples,forobtainingdataconcerningtheadverseeffectsofatestmaterial(addedtodilutionwater)onoceanicbioluminescentdinoflagellates.1.1.1Theendpointforbothproceduresisbasedonameasurablereductionorinhibitioninlightoutputfromthedinoflagellates.Bothproceduresaresimilarinthatwhenbioluminescentdinoflagellatesareexposedtotoxicants,ameasurablereductioninbioluminescenceisobservedfromtheircellsfollowingmechanicalstimulationwhencomparedtocontrolcells.Inthefirstprocedure,cellsofthebioluminescentdinoflagellateGonyaulaxpolyedracanbetestedoverarangeofuptosevendaysofexposure(orlonger)toatoxicant.Thesecondprocedureusesanotherspecies,Pyrocystislunula,fora4htest.1.2Bothprocedurescanmeasurethetoxiceffectsofmanychemicals,variousmarineandfreshwatereffluents,antifoulingcoatings,leachates,andsedimentstobioluminescentdi-noflagellates(1-5).3Compoundswithlowwatersolubilitysuchaslargeorganicmoleculesmaybesolubilizedwithmethanol,ethanol,andacetonesolventsfortesting(4)(seeGuideE729).1.3AnIC50inlightoutput(bioluminescence)istherec-ommendedendpoint(1).However,percentinhibitionofbiolu-minescenceisanappropriateendpointinsomecases(5).1.4Othermodificationsoftheseproceduresmightbejusti-fiedbyspecialneedsorcircumstances.Althoughusingappro-priateproceduresismoreimportantthanfollowingprescribedprocedures,resultsoftestsconductedusingunusualproceduresarenotlikelytobecomparabletoresultsofothertests.Comparisonofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingac...
