ASTM_E_2199_-_08e1.pdf

Designation:E2199081Standard Specification forEncarsia formosa Gahan(Hymenoptera:Aphelinidae)1This standard is issued under the fixed designation E2199;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1NOTESection 1.1 was editorially corrected in November 2008.1.Scope1.1 This specification describes a method for determiningwhether the quantity and quality of adult Encarsia formosa ina shipment adhere to quantity and quality specifications.Thetest also allows the purity of shipments to be determined.Included are referenced documents,a description of standardterminology,specifications,and the test method.2.Referenced Documents2.1 ASTM Standards:2E2200 Specification for Information Included with Packag-ing of Multi-Cellular Biological Control Organisms(Withdrawn 2010)33.Terminology3.1 Definitions:3.1.1 critical valuea number that the test statistic iscompared to in order to determine whether the quantity andquality requirement has been met.Critical values depend onthe probability of error that can be tolerated and on the numberof samples used in the test.3.1.2 carda sample unit;pupae of E.formosa are mountedon cardstock strips that are subdivided into cards.3.1.3 package claimthe number of adults expected toemerge from and leave each card.3.1.4 test statisticthe average number of wasps caughtduring the flight test expressed as a percentage of the packageclaim.3.2 Definitions of Terms Specific to This Standard:3.2.1 name of productEncarsia formosa Gahan.3.2.2 preferred host and preyGreenhouse Whitefly,Tri-aleurodes vaporariorum(Westwood).3.2.3 life stage when shippedpupa within the host insect,T.vaporariorum.4.Classification4.1 PhylumArthropoda.4.2 ClassInsecta.4.3 OrderHymenoptera.4.4 FamilyAphelinidae.4.5 GenusEncarsia.4.6 Speciesformosa.5.Quantity and Quality Specification5.1 The test described in this specification is used for boththe quantity and quality assessment.To meet the quantity andquality specification defined by the“package claim,”100%ofthe number of adults should be capable of flight as measured bythe flight test described herein.6.Purity6.1 The purity specification is that shipments,and hencesamples,do not contain species other than E.formosa.TEST METHODDetermining the Number and FlightCapability of E.formosa Adults in a Shipment,andAssessment of Shipment Purity7.Scope7.1 The test describes methods for determining whether thenumber of adult E.formosa that are capable of flight meets orexceeds the package claim.Amethod of assessing the purity ofa shipment is included.8.Summary of Test Method8.1 The quantity of E.formosa per card and their flightability is determined by counting the number of wasps caughton a sticky trap suspended in a test chamber.Three or more test1This specification is under the jurisdiction of ASTM Committee E35 onPesticides,Antimicrobials,and Alternative Control Agents and are the directresponsibility of Subcommittee E35.30 on Natural Multi-Cellular(Metazoan)Biological Control Organisms.Current edition approved April 1,2008.Published May 2008.Originallyapproved in 2002.Last previous edition approved in 2002 as E2199 02.DOI:10.1520/E2199-08E01.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 chambers are used per shipment,each chamber contains onerandomly chosen card with E.formosa pupae.The numbers ofwasps caught on each sticky card are counted and results areused to calculate the average number of wasps per card.Thisaverage is used in combination with the known samplingdistribution and variation in counts to judge whether specifi-cations are met.Live contaminants are identified and recorded.9.Significance and Use9.1 This method was developed to determine that thenumbers of E.formosa supplied in a shipment meet thepackage claim and that wasps at receipt have good flightcapability.The application of this method will ensure astandardized evaluation of the product and judicious decisionsabout product compliance to the package claim.10.Materials10.1 Test chamber,6.5 by 8 cm acrylic tube.10.2 Support wire.10.3 Double-sided dry-stick yellow sticky trap,2.5 by4.0 cm.10.4 Nylon screen,100-mesh.10.5 Clear plastic cling film,see Fig.1.10.6 Headband magnifier,(7 to 10)is optional.11.Test Unit11.1 A single shipment of E.formosa is considered a testunit.12.Pre-Test Conditions12.1 If required,a shipment can be stored for a maximum of24 h at 10 to 15C,60 to 90%relative humidity.1