Designation:E1758−01(Reapproved2015)StandardTestMethodforDeterminationofCarbohydratesinBiomassbyHighPerformanceLiquidChromatography1ThisstandardisissuedunderthefixeddesignationE1758;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.INTRODUCTIONThecarbohydratesmakingupamajorportionofbiomasssamplesarepolysaccharidesconstructedprimarilyofglucose,xylose,arabinose,galactose,andmannosesubunits.Thepolysaccharidespresentinabiomasssamplecanbehydrolyzedtotheircomponentsugarmonomersbysulfuricacidinatwo-stagehydrolysisprocess.Thesemonosaccharidescanthenbequantifiedbyion-moderatedpartitionHPLC.1.Scope1.1Thistestmethodcoversthedeterminationofcarbohy-dratespresentinabiomasssample,expressedasthepercent,bymass,ofeachsugarona105°Cdriedmassbasis.NOTE1—Thepercentsugarmustbecorrectedforthewaterofhydrolysisbeforecalculatingtheactualmasspercentofthepolysaccha-rideintheoriginalbiomasssample.1.2Samplematerialssuitableforthisprocedureincludehardandsoftwoods,herbaceousmaterials(suchasswitchgrassandsericea),agriculturalresidues(suchascornstover,wheatstraw,andbagasse),wastepaper(suchasofficewaste,boxboard,andnewsprint),acidoralkaline-pretreatedbiomass(washedfreeofanyresidualacidoralkali),andthesolidfractionoffermentationresidues.Allresultsarereportedrelativetothe105°Coven-driedmassofthesample.1.3Theoptionsforthetypesofsamplestobeanalyzedinthistestmethodareasfollows:1.3.1PreparedBiomassSamples:1.3.1.1AirDried(%Tad)—Thepercent,bymass,oftotalsolidsoftheair-driedsample.1.3.1.245°CDried(%T45)—Thepercent,bymass,oftotalsolidsofthe45°Cdriedsample.1.3.1.3FreezeDried(%Tfd)—Thepercent,bymass,oftotalsolidsofthefreezedriedsample.1.3.2Extractives-FreeSample(%Text)—Thepercent,bymass,oftotalsolidsoftheextractedsampledeterminedat105°C.1.4ThevaluesstatedinSIunitsaretoberegardedasthestandard.1.5T...
