Designation:E1687−10(Reapproved2014)AnAmericanNationalStandardStandardTestMethodforDeterminingCarcinogenicPotentialofVirginBaseOilsinMetalworkingFluids1ThisstandardisissuedunderthefixeddesignationE1687;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1ThistestmethodcoversamicrobiologicaltestprocedurebasedupontheSalmonellamutagenesisassayofAmesetal(1)2(seealsoMaronetal(2)).Itcanbeusedasascreeningtechniquetodetectthepresenceofpotentialdermalcarcino-gensinvirginbaseoilsusedintheformulationofmetalwork-ingoils.Personswhoperformthistestshouldbewell-versedintheconductoftheAmestestandconversantwiththephysicalandchemicalpropertiesofpetroleumproducts.1.2Thetestmethodisnotrecommendedasthesoletestingprocedureforoilswhichhaveviscositieslessthan18cSt(90SUS)at40°C,orforformulatedmetalworkingfluids.1.3ThevaluesstatedinSIunitsaretoberegardedasthestandard.Thevaluesgiveninparenthesesareprovidedforinformationonly.1.4Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.Section7providesgeneralguidelinesforsafeconductofthistestmethod.2.ReferencedDocuments2.1ASTMStandards:3E2148GuideforUsingDocumentsRelatedtoMetalworkingorMetalRemovalFluidHealthandSafetyE2523TerminologyforMetalworkingFluidsandOpera-tions2.2OtherStandards:29CFR1910.1450OccupationalExposuretoHazardousChemicalinLaboratories43.Terminology3.1DefinitionsofTermsSpecifictoThisStandard:(SeealsoTerminologyE2523.)3.1.1basestock,n—therefinedoilcomponentofmetal-workingfluidformulations.3.1.2PAC(PolycyclicAromaticCompounds),n—Forthepurposesofthistestmethod,PACreferstofused-ringpolycy-clicaromaticcompoundswiththreeormorerings.Forexampl...
