Designation:E1357–90(Reapproved2001)StandardTestMethodforDeterminingtheRateofBioleachingofIronFromPyritebyThiobacillusFerrooxidans1ThisstandardisissuedunderthefixeddesignationE1357;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethodcoversproceduresfordeterminingtherateofbioleachingofironfrompyrite(FeS2)bythebacteriumThiobacillusferrooxidans.1.2Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:D516TestMethodsforSulfateIoninWater2D1068TestMethodsforIroninWater2D1193SpecificationforReagentWater2D4455TestMethodforEnumerationofAquaticBacteriabyEpifluorescenceMicroscopyCountingProcedure33.Terminology3.1Definition:3.1.1solubleiron—thecomplexedanddissolvedironasdeterminedbyVuorinenetal.4intheirstudyofthespeciesofironreleasedfrompyriteoxidationbyT.ferrooxidans.Theyfoundthatvaluesofcomplexedanddissolvedironcorre-spondedcloselywith“totaliron”asdeterminedafterhotsulfuricaciddigestionofsamples,particularlyat1to2%pulpdensity.4.SummaryofTestMethod4.1CellsofT.ferrooxidansgrownonferrousironareaddedtoconicalflaskscontainingfinelygroundironpyriteinaninorganicsaltsmedium(2%pulpdensity).Thecultureisincubatedwithagitationandsamplesareperiodicallywith-drawnfordeterminationofsolubleiron.Therateofpyriteleachingisdeterminedfromthelinearportionofacurve-plottingsolubleironproducedversustime.4.2Theaveragerateofsolubleironproductioninmgofiron/L/hisreportedalongwithvaluesforuninoculatedcon-trols.Thestandarddeviationfortriplicateflasksisalsoreported.AlsotobereportedistheparticlesizerangeofthepyriteandtheinitialandfinalpHvaluesofthetestsolution...
