Designation:E1203–98(Reapproved2004)StandardPracticeforUsingBrineShrimpNaupliiasFoodforTestAnimalsinAquaticToxicology1ThisstandardisissuedunderthefixeddesignationE1203;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispracticedescribesproceduresforhatching,harvest-ing,andtestingtheacceptabilityofbrineshrimpnaupliiforuseasafoodforcertainfishandinvertebratespeciesthatareusedinaquatictoxicitytests.Theterm“brineshrimp”referstoallspeciesinthegenusArtemia(1)2althoughthispracticespecificallydealsonlywiththosespeciesforwhichcysts(encystedembryos)arecommerciallyavailable.1.2Theseproceduresareapplicabletoallbrineshrimpnaupliithatareobtainedbyincubatingcommerciallyavailablecysts.Withappropriateprocessing,cystscollectedbynoncom-mercialharvesterscanbesubjectedtothesesameprocedures.1.3Modificationoftheseproceduresmightbejustifiedbyspecialneedsorcircumstances.1.4Thispracticeisorganizedasfollows:SectionReferencedDocuments2Terminology3SummaryofPractice4SignificanceandUse5Hazards6ObtainingCysts7SelectingaSource7.1Storage7.2HatchingCysts8HarvestingNauplii9TestingNauplii10FeedingAssay10.1ChemicalandPhysicalMeasurements10.2UsingNauplii11Report121.5Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.SpecifichazardstatementsaregiveninSection6.2.ReferencedDocuments2.1ASTMStandards:3E1191GuideforConductingLife-CycleToxicityTestswithSaltwaterMysidsE1241GuideforConductingEarlyLife-StageToxicityTestswithFishes3.Terminology3.1Thewords“must,”“should,”“may,”“can,”and“might”haveveryspecificmeaningsinthispractice.“Must”isusedtoexpressanabsoluterequirement,thatis,tostatethatthetesto...
