Designation:E1366−11StandardPracticeforStandardizedAquaticMicrocosms:FreshWater1ThisstandardisissuedunderthefixeddesignationE1366;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispracticecoversproceduresforobtainingdataconcerningtoxicityandothereffectsofatestmaterialtoamulti-trophiclevelfreshwatercommunity.1.2Theseproceduresalsomightbeusefulforstudyingthefateoftestmaterialsandtransformationproducts,althoughmodificationsandadditionalanalyticalproceduresmightbenecessary.1.3Modificationoftheseproceduresmightbejustifiedbyspecialneedsorcircumstances.Althoughusingappropriateproceduresismoreimportantthanfollowingprescribedprocedures,resultsoftestsconductedusingunusualproceduresarenotlikelytobecomparabletoresultsofmanyothertests.Comparisonofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingmulti-trophicleveltests.1.4Thispracticeisarrangedasfollows:SectionReferencedDocuments2Terminology3SummaryofPractice4SignificanceandUse5Apparatus6Facilities6.1Container6.2Equipment6.3Hazards7MicrocosmComponents8Medium8.1MediumPreparation8.2Sediment8.3MicrocosmAssembly8.4TestMaterial9General9.1StockSolution9.2NutrientControl9.3TestConcentration(s)9.4TestOrganisms10Algae10.1Animals10.2SpecificityofOrganisms10.3Sources10.4AlgalCultureMaintenance10.5AnimalCultureMaintenance10.6SectionProcedure11ExperimentalDesign11.1ProcedureInoculation11.2Culling11.3AdditionofTestMaterial11.4Measurements11.5Reinoculations11.6AnalyticalMethodology12DataProcessing13CalculationsofVariablesfromMeasurements14StatisticalAnalyses15AcceptabilityofTest16InterpretationofResults17Report18AnnexAnnexA1AppendicesRelationshipofMediaAppendixX1StatisticalGuidanceAppendixX21.5ThevaluesstatedinSIunitsaretoberegarded...
