Designation:E1163−10(Reapproved2016)StandardTestMethodforEstimatingAcuteOralToxicityinRats1ThisstandardisissuedunderthefixeddesignationE1163;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddeterminesthelethality(LD50value,slopeand95%confidenceinterval(CI))andsignsofacutetoxicityfromamaterialusingalimitednumberofrats.Thetechniqueusedinthistestmethodisreferredtoasthe“Stagewise,AdaptiveDoseMethod.”2ThistestmethodisanalternativetotheclassicalLD50testandisapplicabletobothliquidsandsolids.1.2Thistestmethodisnotrecommendedfortestmaterialswhichtypicallyproducedeathsbeyondtwodayspostdosing.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:3E609TerminologyRelatingtoPesticidesIEEE/ASTMSI10StandardforUseoftheInternationalSystemofUnits(SI)(theModernizedMetricSystem)3.Terminology3.1Definitions:3.1.1delayeddeath—ananimalwhichdoesnotdieorappearmoribundwithin24hbutdieslaterduringtheobser-vationperiod.3.1.2gavage—forcedoraldosing,asbyatubethatispasseddownthethroattothestomach.3.1.3LD50—thestatisticallyderivedestimateofthedoseofatestsubstancethatwouldbeexpectedtocause50%mortalitytothetestpopulationunderthespecifiedtestconditions.3.1.4moribund—atthepointofdeathorextinction.3.1.5pharmacotoxic—grossphysiologicalsignsinresponsetoatoxicmaterial.3.1.6signsoftoxicity—objective,observableevidenceoftoxicity.3.1.7suspension—amixtureinwhichverysmallparticlesremainsuspendedwithoutdissolving.3.1.8toxicity—poisonousquality.4.SummaryofTestMethod4.1Threetofivedifferentdosesofthetargetcompoundareselectedsuchthatthedosesspantheentired...
