Designation:E1279–89(Reapproved2001)StandardTestMethodforBiodegradationByaShake-FlaskDie-AwayMethod1ThisstandardisissuedunderthefixeddesignationE1279;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(e)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestmethoddescribesproceduresforassessingthebiodegradationofchemicalsinnaturalsurfacewatersamples.1.2Thistestmethodprovidesanopportunitytoevaluateratesofbiodegradationinthepresenceandabsenceofnaturalsedimentmaterials.Italsomayprovidelimitedinformationontheabioticdegradationrate,andsorptiontosedimentandvesselwalls.1.3Thistestmethodallowsforthedevelopmentofafirst-orderrateconstant,basedonthedisappearanceofthetestcompoundwithtime,andasecond-orderrateconstant,nor-malizedforchangesinmicrobialbiomass.1.4Thistestmethodrequiresachemicalspecificanalyticalmethodandtheconcentrationsoftestsubstanceemployedaredependentonthesensitivityoftheanalyticalmethod.1.5Thistestmethodisdesignedtobeapplicabletocom-poundsthatarenotinhibitorytobacteriaattheconcentrationsusedinthetestmethod,whichdonotrapidlyvolatilizefromwater,thataresolubleattheinitialtestconcentrationandthatdonotdegraderapidlybyabioticprocesses,suchashydrolysis.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:D1193SpecificationforReagentWater2D4129TestMethodforTotalandOrganicCarboninWaterbyOxidationandCoulometricDetection3E895PracticeforDeterminationofHydrolysisRateCon-stantsofOrganicChemicalsinAqueousSolutions4E896TestMethodforConductingAqueousDirectPho-tolysisTests4E1194TestMethodforVaporPressure4E1195TestMethodforDeterminingaSorptionConstant43.SummaryofTestMethod3.1Theshake-flaskd...
