Designation:E1498−92(Reapproved2012)StandardGuideforConductingSexualReproductionTestswithSeaweeds1ThisstandardisissuedunderthefixeddesignationE1498;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidecoversproceduresforobtaininglaboratorydataconcerningtheadverseeffectsofatestmaterialaddedtodilutionwateronsexualreproductionbyseaweeds.Theexposuredurationisspeciesdependentandisfollowedbyaperiodofdevelopmenttoallowtheevidenceofsexualrepro-ductiontoappear.Thereisnoexposuretotoxicantsduringthedevelopmentperiod.Thisrestrictsthetestsprimarilytotheeventssurroundingeggfertilization,anditminimizesanytimelageffectsondevelopmentthatmightinterferewithcorrectenumerationofthenumberofsexualeventsthatoccurred.Theseprocedureswillprobablybeusefulforcon-ductingsexualreproductiontoxicitytestswithavarietyofspeciesofseaweeds,althoughmodificationsmightbeneces-sary.1.2Othermodificationsoftheseproceduresmightbejusti-fiedbyspecialneedsorcircumstances.Althoughusingappro-priateproceduresismoreimportantthanfollowingprescribedprocedures,theresultsoftestsconductedusingunusualpro-ceduresarenotlikelytobecomparabletothoseofmanyothertests.Comparisonoftheresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingsexualreproductiontestswithseaweeds.1.3Theseproceduresareapplicabletomostchemicals,eitherindividuallyorinformulations,commercialproducts,andknownmixturesorwholeeffluents,aswellasforuseintestingsurfacewaters.Withappropriatemodifications,theseprocedurescanbeusedtostudytheeffectsoftemperature,dissolvedoxygen,pH,andsuchmaterialsasleachates,oils,particulatematter,andsediments.1.4ThevaluesstatedinSIunitsaretoberegardedasthestandard.1.5Thisguideisarrangedasfollows:SectionReferencedDocuments2Termin...
