Designation:E1199−87(Reapproved2012)StandardPracticeforSamplingZooplanktonwithaClarke-BumpusPlanktonSampler1ThisstandardisissuedunderthefixeddesignationE1199;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispracticecoverstheproceduresforobtainingquan-titativesamplesofazooplanktoncommunitybyuseofaClarke-Bumpusplanktonsampler.1.2Thisstandarddoesnotpurporttoaddressallofthesafetyproblems,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2D4134PracticeforSamplingPhytoplanktonwithaClarke-BumpusPlanktonSamplerE1200PracticeforPreservingZooplanktonSamples3.SummaryofPractice3.1Thesampleristowedfromamovingboatataspecifieddepth.Thesamplerusesanetforthecollectionandconcen-trationofzooplankton.Theactualvolumeofwaterenteringthesamplerismeasuredbyacalibratedflowmeter.Thezooplank-tonarepreservedasdictatedbytheobjectivesofthestudy.4.SignificanceandUse4.1TheadvantagesoftheClarke-Bumpusplanktonsamplerareasfollows:4.1.1Itwillsampleadiscretedepthormultipledepths,dependinguponthesamplingdesign.4.1.2Itisaslowtomediumspeedsamplerrequiringatowingspeedofthreetofiveknots.4.1.3Thesamplesizecanbeeasilycontrolled.4.1.4Thesamplerislightweightandcanbeusedwithoutauxiliaryequipment.4.1.5Ithasarelativelyhighfiltrationefficiencyfactorof0.88.4.1.6Itisaversatilesamplerandcanbeusedinallbuttheshallowestwaters.4.1.7Theflowmeterrecordstheamountofwaterthatpassesintothenet.4.1.8Overspillofwateratthemouthofthenetduetoexcessspeedoftowingisofminimalconsequence.4.2ThedisadvantagesoftheClarke-Bumpusplanktonsam-plerareasfollows:4.2.1Theflowmeterrequiresfrequentmaintenanceinclud-ingcalibrationandlubrication.4.2.2Itisnotsuitablefo...