ASTM_E_1241_-_05_2013.pdf

Designation:E124105(Reapproved 2013)Standard Guide forConducting Early Life-Stage Toxicity Tests with Fishes1This standard is issued under the fixed designation E1241;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This guide describes procedures for obtaining laboratorydata concerning the adverse effects of a test material added todilution waterbut not to foodon certain species of fresh-water and saltwater fishes during 28 to 120-day(depending onspecies)continuous exposure,beginning before hatch andending after hatch,using the flow-through technique.Thisguide will probably be useful for conducting early life-stagetoxicity tests with some other species of fish,although modi-fications might be necessary.1.2 Other modifications of these procedures might be justi-fied by special needs or circumstances.Although using appro-priate procedures is more important than following prescribedprocedures,results of tests conducted using unusual proceduresare not likely to be comparable to results of many other tests.Comparison of results obtained using modified and unmodifiedversions of these procedures might provide useful informationconcerning new concepts and procedures for conducting earlylife-stage toxicity tests with fishes.1.3 These procedures are applicable to all chemicals,eitherindividually or in formulations,commercial products,orknown mixtures,that can be measured accurately at thenecessary concentrations in water.With appropriate modifica-tions these procedures can be used to conduct tests ontemperature,dissolved oxygen,and pH and on such materialsas aqueous effluents(see Guide E1192),leachates,oils,par-ticulate matter,sediments,and surface waters.1.4 This guide is arranged as follows:SectionReferenced Documents2Terminology3Summary of Standard4Significance and Use5.1Hazards6Apparatus7Facilities7.1Construction Materials7.2Metering System7.3Test Chambers and Incubation Cups7.4Cleaning7.5Acceptability7.6Dilution Water8Requirements8.1Source8.2Treatment8.3Characterization8.4Test Material9General9.1Stock Solution9.2Test Concentration(s)9.3Test Organisms10Species10.1Age10.2Source10.3Brood Stock10.4Handling10.5Procedure11Experimental Design11.1Dissolved Oxygen11.2Temperature11.3Beginning the Test11.4Thinning11.5Feeding11.6Duration of Test11.7Biological Data11.8Other Measurements11.9Analytical Methodology12Acceptability of Test13Calculation of Results14Documentation15AppendixesAppendix X1 Salmon,Trout,and CharAppendix X2 Northern pikeAppendix X3 Fathead minnowAppendix X4 White suckerAppendix X5 Channel catfishAppendix X6 Bluegill1This guide is under the jurisdiction ofASTM Committee E50 on EnvironmentalAssessment,Risk Management and Corrective Action and is the direct responsibil-ity of Subcommittee E50.47 on Biological Effects and Environmental Fate.Current edition approved March 1,2013.Published March 2013.Originallyapproved in 1988.Last previous edition approved in 2005 as E1241 05.DOI:10.1520/E1241-05R13.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 Appendix X7 Gulf toadfishAppendix X8 Sheepshead minnowAppendix X9 SilversidesAppendix X10 Statistical GuidanceAppendix X11.Striped Bass1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.Specific hazardstatements are given in Section 6 and 9.2.Referenced Documents2.1 ASTM Standards:2E380 Practice for Use of the International System of Units(SI)(the Modernized Metric System)(Withdrawn 1997)3E729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating to Biological Effects and Envi-ronmental FateE1023 Guide for Assessing the Hazard of a Material toAquatic Organisms and Their UsesE1192 Guide for Conducting Acute Toxicity Tests on Aque-ous Ambient Samples and Effluents with Fishes,Macroinvertebrates,and AmphibiansE1203 Practice for Using Brine Shrimp Nauplii as Food forTest Animals in Aquatic Toxicology(Withdrawn 2013)33.Terminology3.1 The words“must,”“should,”“may,”“can,”and“might”have very specific meanings in this standard.“Must”is used toexpress an absolute requirement,that is,to state that the testought to be designed to satisfy the specified condition,unlessthe purpose of the test requires a different design.“Must”isonly used in connection with factors that directly relate to theacceptability of the test(see 13.1).“Should”is used to statethat the specified condition is recommended and ought to bemet if possible.Although violation of one“should”is rarely