ASTM_E_193_-_94_2010.pdf

Designation:E19394(Reapproved 2010)Standard Specification forLaboratory Glass Micropipets1This standard is issued under the fixed designation E193;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This specification covers several micropipets useful inmeasuring microlitre quantities of liquids.NOTE1This specification was originally developed by the Committeeon MicrochemicalApparatus,Division ofAnalytical Chemistry,AmericanChemical Society.2,3,41.2 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.2.Referenced Documents2.1 ASTM Standards:5E920 Specification for Commercially Packaged LaboratoryApparatusE921 Specification for Export Packaged Laboratory Appa-ratusE1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United States Government Procure-mentsE1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3.Nomenclature3.1 The pipets specified in Figs.1-3,and Fig.4 are classifiedas micropipets,whereas those in Figs.5 and 6 are classified asmicrolitre pipets in order to differentiate among the variouspipets,not on a technical basis,but rather on the basis of usage.This is done to prevent confusion that might result from thefact that the pipets shown in Figs.1 and 2,and Fig.3 are listedin trade catalogs as micropipets designed especially for use inthe fields of biological and clinical chemistry,and the pipetsshown in Fig.4 are listed as micropipets,density-type,de-signed for use as microweighing pipets,or pycnometers.4.Micropipets,Measuring-Type(“to deliver”)4.1 The measuring-type micropipets2,6are specified in fivecapacities and shall conform to the requirements shown in Fig.1.The narrow,elongated delivery stem of the pipets can reachto the bottom of microvolumetric flasks,2,5permitting almostcomplete withdrawal of the contents of the flask.Quantitativedelivery of the entire volume of the pipets is obtained bytouching off the last drop.5.Micropipets,Folin-Type(“to contain”)5.1 The Folin-type4,6,7micropipets are specified in twocapacities and shall conform to the requirements shown in Fig.2.Complete delivery of the volume indicated by the graduationmark on these pipets is ensured by washing out several timesany solution adhering to the inner surface with wash liquiddrawn up from the tip.6.Micro Washout Pipets,Pregl-Type(“to contain”)6.1 The micro washout pipets,Pregl-type4,6,8are specifiedin four capacities and shall conform to the requirements shownin Fig.3.Quantitative delivery of the volume indicated by thegraduation mark on the pipets is obtained by rinsing out thecontents with wash liquid added from the top of the pipet.7.Micro Weighing Pipets,Density-Type(Pycnometers)(“to contain”)7.1 The micro weighing pipets,density-type(pycnometers),4,6,9with ground-glass caps,are specified in three capacities1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved July 1,2010.Published July 2010.Originally approvedin 1962.Last previous edition approved in 2005 as E193 94(2005).DOI:10.1520/E0193-94R10.2Committee on Microchemical Apparatus,Division of Analytical Chemistry,American Chemical Society.“Report on Recommended Specifications for Micro-chemical Apparatus,Volumetric Glassware,Flasks,Pipets,and Centrifuge Tubes,”Analytical Chemistry,Vol 28,1956,p.1993.3Committee on Microchemical Apparatus,Division of Analytical Chemistry,American Chemical Society.“Report on Recommended Specifications for Micro-chemical Apparatus,Volumetric Glassware,Microlitre Pipets,”AnalyticalChemistry,Vol 30,1958,p.1702.4Committee on Microchemical Apparatus,Division of Analytical Chemistry,American Chemical Society.“Report on Recommended Specifications for Micro-chemical Apparatus,Volumetric Glassware,Micropipets,”Analytical Chemistry,Vol 32,1960,p.1045.5For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.6Steyermark,A.,Quantitative Organic Microanalysis,2nd Ed.,Academic Press,New York,NY,1961.7Folin,O.,Journal of Biological Chemistry,Vol 77,1928,p.421.8Grant,J.,Quantitative Organic Microanalysis Based on Methods of Fritz Pregl,5th Ed.,Blakiston Co.,Philadelphia,PA 1951,p.34.9Alber,H.K.,Industrial and Engineering Chemistry,Analytical Edition,Vol 12,1940,p.764.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 and shall conform to the requirements shown in Fig.4.Thesepipets are designed for density determinations on smallamo