Designation:E1841−04(Reapproved2012)StandardGuideforConductingRenewalPhytotoxicityTestsWithFreshwaterEmergentMacrophytes1ThisstandardisissuedunderthefixeddesignationE1841;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thistestguideisdesignedtogivegeneralguidanceforassessingthepotentialphytotoxicityofwatersolubletestmaterialtofreshwateremergentmacrophytes.1.2Thisrenewaltestcontinuouslyexposesselectedplantspecies,growinginsediment,tovariousconcentrationsoftestmaterial,dissolvedinanutrientsolution.1.3ThistestguideisbasedontheToxicSubstancesControlAct(TSCA)guidelinesforconductingtoxicitytestswithterrestrialplants(1)2andisapplicabletomostwatersolublechemicals,eitherindividuallyorinformulations,commercialproducts,orknownmixtures(seeGuidesE1193andE1598).Withslightmodificationstheprocedurealsomightbeusedforeffluents(seeGuideE1192).1.4ResultsfromthistoxicitytestcanbeusedtoreportanIC50orNOEC(seeSection3)basedontheconcentrationofchlorophyllextractedfromtheplants(seeGuidesD3731andE1218).Insomesituations,itmightbenecessarytoonlytestatoneconcentrationtodeterminewhetherornotthatspecificconcentrationistoxictotheplants.1.5Thistestmethodisarrangedasfollows:SectionReferencedDocuments2Terminology3SummaryofGuide4SignificanceandUse5Hazards7ApparatusandReagents6Facilities6.1TestChambers6.2Cleaning6.3HPLC6.4Reagents6.5NutrientSolution8TestMaterial9General9.1TestConcentrations9.2StockSolution9.3Controls9.4Sediments10TestOrganisms11RecommendedSpecies11.1AlternateSpecies11.2Culturing11.3Procedure12ExperimentalDesign12.1BeginningofTest12.2EvaluationofTest12.3Calculation13AcceptabilityofTest14Report15PrecisionandBias16Keywords17AppendixX1References1.6ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.7Thisstandarddoesnotpur...
