Designation:E1562−00(Reapproved2013)StandardGuideforConductingAcute,Chronic,andLife-CycleAquaticToxicityTestswithPolychaetousAnnelids1ThisstandardisissuedunderthefixeddesignationE1562;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thisguidecoversproceduresforobtainingdatacon-cerningtheadverseeffectsofatestmaterialaddedtomarineandestuarinewatersoncertainspeciesofpolychaetesduringshort-orlong-termcontinuousexposure.Thepolychaetespeciesusedinthesetestsaretakenfromlaboratoryculturesandexposedtovaryingconcentrationsofatoxicantinstaticconditions.Theseproceduresmaybeusefulforconductingtoxicitytestswithotherspeciesofpolychaetes,althoughmodificationsmightbenecessary.1.2Modificationsoftheseproceduresmightbejustifiedbyspecialneedsorcircumstances.Althoughusingappropriateproceduresismoreimportantthanfollowingprescribedprocedures,theresultsoftestsconductedusingunusualpro-ceduresarenotlikelytobecomparabletothoseofmanyothertests.Comparisonsofresultsobtainedusingmodifiedandunmodifiedversionsoftheseproceduresmightprovideusefulinformationconcerningnewconceptsandproceduresforconductingacute,chronic,orlife-cycletestswithotherspeciesofpolychaetes.1.3Theseproceduresareapplicabletomostchemicals,eitherindividuallyorinformulations,commercialproducts,andknownorunknownmixtures.Withappropriatemodifications,theseprocedurescanbeusedtoconductthesetestsonfactorssuchastemperature,salinity,anddissolvedoxygen.Theseprocedurescanalsobeusedtoassessthetoxicityofpotentiallytoxicdischargessuchasmunicipalwastes,oildrillingfluids,producedwaterfromoilwellproduction,andothertypesofindustrialwastes.AnLC50(mediallethalconcentration)iscalculatedfromthedatageneratedineachacuteandchronictoxicitytest.Reproductivesuccessandthenumberofoffspringproducedareusedtomeasuretheeffectofatoxicantonlife-cycle...
