ASTM_E_1562_-_00_2013.pdf

Designation:E156200(Reapproved 2013)Standard Guide forConducting Acute,Chronic,and Life-Cycle Aquatic ToxicityTests with Polychaetous Annelids1This standard is issued under the fixed designation E1562;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This guide covers procedures for obtaining data con-cerning the adverse effects of a test material added to marineand estuarine waters on certain species of polychaetes duringshort-or long-term continuous exposure.The polychaetespecies used in these tests are taken from laboratory culturesand exposed to varying concentrations of a toxicant in staticconditions.These procedures may be useful for conductingtoxicity tests with other species of polychaetes,althoughmodifications might be necessary.1.2 Modifications of these procedures might be justified byspecial needs or circumstances.Although using appropriateprocedures is more important than following prescribedprocedures,the results of tests conducted using unusual pro-cedures are not likely to be comparable to those of many othertests.Comparisons of results obtained using modified andunmodified versions of these procedures might provide usefulinformation concerning new concepts and procedures forconducting acute,chronic,or life-cycle tests with other speciesof polychaetes.1.3 These procedures are applicable to most chemicals,either individually or in formulations,commercial products,andknownorunknownmixtures.Withappropriatemodifications,these procedures can be used to conduct thesetests on factors such as temperature,salinity,and dissolvedoxygen.These procedures can also be used to assess thetoxicity of potentially toxic discharges such as municipalwastes,oil drilling fluids,produced water from oil wellproduction,and other types of industrial wastes.An LC50(medial lethal concentration)is calculated from the datagenerated in each acute and chronic toxicity test.Reproductivesuccess and the number of offspring produced are used tomeasure the effect of a toxicant on life-cycle tests;data areanalyzed statistically to indicate that concentration at which asignificant difference occurs between the test solutions andcontrol(s).1.4 The results of acute or chronic toxicity tests withtoxicants added experimentally to salt water should usually bereported in terms of an LC50.The results of life-cycle toxicitytests with toxicants added experimentally to salt water shouldbe reported as that concentration at which a statisticallysignificant difference in the number of offspring is producedwith reference to the control(s).1.5 This guide is arranged as follows:SectionReferenced Documents2Terminology3Summary of Guide4Significance and Use5Apparatus6Facilities6.1Construction Materials6.2Test Chambers6.3Cleaning6.4Acceptability6.5Safety Precautions7Dilution Water8Requirements8.1Source8.2Treatment8.3Characterization8.4Test Material9General9.1Stock Solution9.2Test Concentrations9.3Test Organisms10Species10.1Age10.2Source10.3Feeding10.4Holding10.5Quality10.6Procedure11Experimental Design11.1Acute Test11.1.1Chronic Test11.1.2Life-Cycle Test11.1.3Test Condition Specifications11.2Dissolved Oxygen11.2.1Temperature11.2.2Loading11.2.3Salinity11.2.4Light11.2.5Beginning the Test11.3Feeding11.4Duration of Test11.5Biological Data11.6Other Measurements11.7Analytical Methodology121This guide is under the jurisdiction ofASTM Committee E50 on EnvironmentalAssessment,Risk Management and CorrectiveActionand is the direct responsibilityof Subcommittee E50.47 on Biological Effects and Environmental Fate.Current edition approved March 1,2013.Published March 2013.Originallyapproved in 1994.Last previous edition approved in 2006 as E1562 00(2006).DOI:10.1520/E1562-00R13.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 Acceptability of Test13Calculation of Results14Report15Keywords16Appendixes:Neanthes arenaceodentataAppendix X1Capitella capitataAppendix X2Ophryotrocha diademaAppendix X3Dinophilus gyrociliatusAppendix X41.6 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.7 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.Specific precau-tionary statements are given in Section 7.2.Referenced Documents2.1 ASTM Standards:2E729 Guide for Conducting Acute Toxicity Tests on TestMaterials with Fishes,Macroinvertebrates,and Amphib-iansE943 Terminology Relating to Biological Effects and Envi-ronmental FateE1023 Guide for Assessing the Hazard of a Material toAqua