ASTM_E_1259_-_16.pdf

Designation:E125916Standard Practice forEvaluation of Antimicrobials in Liquid Fuels Boiling Below390C1This standard is issued under the fixed designation E1259;the number immediately following the designation indicates the year oforiginal adoption or,in the case of revision,the year of last revision.A number in parentheses indicates the year of last reapproval.Asuperscript epsilon()indicates an editorial change since the last revision or reapproval.1.Scope1.1 This practice is designed to evaluate antimicrobialagents for the prevention of microbially influenced deteriora-tion of liquid fuels(as defined by Specification D396,D910,D975,D1655,D2069,D2880,D3699,D4814,D6227,D6751,and D7467),system deterioration,or both.1.2 Knowledge of microbiological techniques is requiredfor these procedures.1.3 It is the responsibility of the investigator to determinewhether Good Laboratory Practice(GLP)is required and tofollow them where appropriate(40 CFR,160),or as revised.1.4 The values stated in SI units are to be regarded asstandard.No other units of measurement are included in thisstandard.1.5 This standard does not purport to address all of thesafety concerns,if any,associated with its use.It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.2.Referenced Documents2.1 ASTM Standards:2D396 Specification for Fuel OilsD910 Specification for Leaded Aviation GasolinesD975 Specification for Diesel Fuel OilsD1655 Specification for Aviation Turbine FuelsD2069 Specification for Marine Fuels(Withdrawn 2003)3D2880 Specification for Gas Turbine Fuel OilsD3699 Specification for KerosineD4814 Specification for Automotive Spark-Ignition EngineFuelD5465 Practice for Determining Microbial Colony Countsfrom Waters Analyzed by Plating MethodsD6227 Specification for Unleaded Aviation Gasoline Con-taining a Non-hydrocarbon ComponentD6293 Test Method for Oxygenates and Paraffin,Olefin,Naphthene,Aromatic(O-PONA)Hydrocarbon Types inLow-Olefin Spark Ignition Engine Fuels by Gas Chroma-tography(Withdrawn 2009)3D6469 Guide for Microbial Contamination in Fuels and FuelSystemsD6729 Test Method for Determination of Individual Com-ponents in Spark Ignition Engine Fuels by 100 MetreCapillary High Resolution Gas ChromatographyD6733 Test Method for Determination of Individual Com-ponents in Spark Ignition Engine Fuels by 50-MetreCapillary High Resolution Gas ChromatographyD6751 Specification for Biodiesel Fuel Blend Stock(B100)for Middle Distillate FuelsD6974 Practice for Enumeration of Viable Bacteria andFungi in Liquid FuelsFiltration and Culture ProceduresD7463 Test Method forAdenosine Triphosphate(ATP)Con-tent of Microorganisms in Fuel,Fuel/Water Mixtures,andFuel Associated WaterD7464 Practice for Manual Sampling of Liquid Fuels,As-sociated Materials and Fuel System Components forMicrobiological TestingD7467 Specification for Diesel Fuel Oil,Biodiesel Blend(B6 to B20)D7687 Test Method for Measurement of Cellular AdenosineTriphosphate in Fuel,Fuel/Water Mixtures,and Fuel-Associated Water with Sample Concentration by FiltrationD7978 Test Method for Determination of the ViableAerobicMicrobial Content of Fuels and Associated WaterThixotropic Gel Culture MethodE1259 Practice for Evaluation of Antimicrobials in LiquidFuels Boiling Below 390CE1326 Guide for Evaluating Non-culture MicrobiologicalTests1This practice is under the jurisdiction of ASTM Committee E35 on Pesticides,Antimicrobials,and Alternative Control Agents and is the direct responsibility ofSubcommittee E35.15 on Antimicrobial Agents.Current edition approved April 1,2016.Published May 2016.Originallyapproved in 1988.Last previous edition approved in 2010 as E1259 10.DOI:10.1520/E1259-16.2For referenced ASTM standards,visit the ASTM website,www.astm.org,orcontact ASTM Customer Service at serviceastm.org.For Annual Book of ASTMStandards volume information,refer to the standards Document Summary page onthe ASTM website.3The last approved version of this historical standard is referenced onwww.astm.org.Copyright ASTM International,100 Barr Harbor Drive,PO Box C700,West Conshohocken,PA 19428-2959.United States1 2.2 NACE Standard:TM0172 Determining Corrosive Properties of Cargoes inPetroleum Product Pipelines42.3 Federal Standards:40 CFR Part 79 Fuels and Fuel Additives RegistrationRegulations540 CFR Part 152 Pesticide Registration and ClassificationProcedures53.Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 antimicrobial,nsee biocide.3.1.2 biocide,na physical or chemical agent that killsliving organisms.3.1.2.1 DiscussionBiocides are further classified as bac-tericides(kill bacteria),fungicides(kill fungi),and microbi-cides(kill both bacterial and fungi).They are also referred toas antimicrobials.3.1.3 microbially-influenced deterioration,ndecomposition/degradation of material(fuel)or makingunsuitable for use,as a result of metabolic activity or thepre