Designation:E1482−12StandardPracticeforUseofGelFiltrationColumnsforCytotoxicityReductionandNeutralization1ThisstandardisissuedunderthefixeddesignationE1482;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.ScopeNOTE1—ThetitlewasformerlyStandardTestMethodforNeutraliza-tionofVirucidalAgentsinVirucidalEfficacyEvaluations.1.1Thispracticeisintendedtobeusedtoreducethecytotoxiclevelofthevirus-testproductmixturepriortoassayingforviralinfectivity.Itisusedinconjunctionwithevaluationsofthevirucidalefficacyofdisinfectantsolutions,wipes,triggersprays,orpressurizeddisinfectantsprayprod-uctsintendedforuseoninanimate,nonporousenvironmentalsurfaces.Thispracticemayalsobeusedintheevaluationofhygienichandwashes/handrubs,orforotherspecialapplica-tions.Thepracticemaybeemployedwithallvirusesandhostsystems.1.2Thispracticeshouldbeperformedonlybypersonstrainedinvirologytechniques.1.3Thispracticeutilizesgelfiltrationtechnology.Theeffectivenessofthepracticeisdependentontheratioofgelbedvolumetosamplesizeanduniformityinthepreparationofcolumnsaswellastheconditionsofentrifugation.Theeffectivenessofthispracticeismaximizedbyinvestigatorpracticeandexperiencewithgelfiltrationtechniques.1.4Thispracticewillaidinthereduction,butnotnecessar-ilyelimination,oftestproducttoxicitywhilepreservingthetiteroftheinputvirus.1.5ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafetyandhealthpracticesanddeterminetheapplica-bilityofregulatorylimitationspriortouse.2.ReferencedDocuments2.1ASTMStandards:2E1052TestMethodtoAssesstheActivityofMicrobicidesagainstVirusesinSuspensionE1053TestMethodtoAssessVirucida...
