Designation:D932−20StandardPracticeforFilamentousIronBacteriainWaterandWater-FormedDeposits1ThisstandardisissuedunderthefixeddesignationD932;thenumberimmediatelyfollowingthedesignationindicatestheyearoforiginaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.Asuperscriptepsilon(´)indicatesaneditorialchangesincethelastrevisionorreapproval.1.Scope1.1Thispracticecoversthedeterminationoffilamentousironbacteria(FIB)bymicroscopicexamination.Thispracticeprovidesfortheidentificationofthefollowinggeneraofbacteriafoundinwaterandwater-formeddeposits:Siderocapsa,Gallionella(Dioymohelix),Sphaerotilus,Crenothrix,Leptothrix,andClonothrix.1.2ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.3Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappro-priatesafety,health,andenvironmentalpracticesanddeter-minetheapplicabilityofregulatorylimitationspriortouse.1.4Thisinternationalstandardwasdevelopedinaccor-dancewithinternationallyrecognizedprinciplesonstandard-izationestablishedintheDecisiononPrinciplesfortheDevelopmentofInternationalStandards,GuidesandRecom-mendationsissuedbytheWorldTradeOrganizationTechnicalBarrierstoTrade(TBT)Committee.2.ReferencedDocuments2.1ASTMStandards:2D887PracticesforSamplingWater-FormedDepositsD1129TerminologyRelatingtoWaterD1193SpecificationforReagentWaterD3370PracticesforSamplingWaterfromFlowingProcessStreamsD5465PracticesforDeterminingMicrobialColonyCountsfromWatersAnalyzedbyPlatingMethods3.Terminology3.1Definitions:3.1.1Fordefinitionsoftermsusedinthisstandard,refertoTerminologyD1129.4.SummaryofPractice4.1Theironbacteriaaregenerallyfilamentous,typicallyfoundinfreshwater,andfrequentlysurroundedbyasheathwhichisusuallyencrustedwithironormanganese,orboth(1,2).3However,Starkey(3)reportsanothertypewhichisclassifiedamongthetruebacteria.Detectionandidentificationisa...
