爬山虎的籽能吃吗【爬山虎籽总黄酮提取及鉴别】[]目的:研究爬山虎中黄酮的鉴别及总黄酮的提取工艺。方法:采用传统溶剂浸提法进行常规的化学定性分析确定爬山虎籽中含有黄酮类化合物,并采用五因素四水平的正交试验优化提取工艺。结果:实验证实爬山虎中含有大量黄酮类化合物。正交实验得出了最正确提取工艺条件:提取温度为50℃,料液比为1∶12,提取时间为3h,提取剂为pH=2的65%乙醇溶液。以芦丁为标准品,用紫外分光光度法测定爬山虎籽中总黄酮含量为2.3%。结论:爬山虎籽中含有丰富的黄酮类化合物,优化并建立了爬山虎籽中总黄酮的提取工艺条件。[关键词]爬山虎籽;黄酮类化合物;定性分析;正交试验;含量测定[中图分类号]R943[文献标识码]A[文章编号]1673-7210(2022)06(c)-065-02ExtractiontechniquesandidentificationofflavonoidsinparthenocissusseedsSHIJun1,HANXuan2,ZHANGYanjun1,SUNTijian1,DIAOHaipeng1,CAOXiaofeng1x(1.DepartmentofChemistry,ShanxiMedicalUniversity,Shanxi030001,China;2.HealthVocational&TechnicalCollege,HebeiUniversity,Baoding071002,China)[Abstract]Objective:Tomakeacomprehensivestudyonidentificationandextractiontechnologyofflavonoidsfromtheparthenocissusseeds.Methods:Traditionalsolventextractionmethodandorthogonalexperimentswereused.Flavonoidsintheparthenocissusseedswereconfirmedbyconventionalqualitativeanalysis.Results:Theresearchshowedthattheparthenocissusseedscontainflavonoids.Theoptimumtechnicalconditionweresummedupasfollowsthroughtheorthogonalexperiments:extractiontemperaturewasat50℃,proportionmaterialandextractingreagentwas1∶12,theextractingtimewas3h,extractingreagentwas65%aqueousethanolsolution,andpH=2.Comparingtoruitinstandard,extractionrateofflavonidsinparthenocissusseedswas2.3%byUVspectrophotometry.Conclusion:Flavonoidsisrichintheparthenocissusseeds.Thepreliminaryextractingtechnologyofflavonoidsfromtheparthenocissusseedsisestablishedinthestudy.[Keywords]Parthenocissusseeds;Flavonoids;Qualitativeanalysis;Orthogonalexperiments;Determination黄酮类化合物是天然药用植物的主要活性成分之一,其众多的生理功能使其在医疗方面应用较为广泛,可以预防高血压,具有保肝、降血脂、降胆固醇等多种功能[1-3]。此外,它还是重要的功能...
