常用蛋白酶酶切位点.docx

Cleavage Sites Table for Fusion Proteins： PROTEASE COMPANY Protease Capture Thrombin Leu-Val-Pro-Arg��Gly-Ser Amersham-Biosciences, Novagen,  SIGMA, Roche Benzamidine-Agarose Factor Xa  Ile-Glu/Asp-Gly-Arg��  Amersham-Biosciences, New England Biolabs,  Roche Benzamidine-Agarose Enterokinase Asp-Asp-Asp-Asp-Lys�� New England Biolabs,  Novagen, Roche Trypsin Inhibitor-Agarose TEV protease Glu-Asn-Leu-Tyr-Phe-Gln��Gly Invitrogen – Life Technologies  Ni-NTA (6His recomb. TEV) PreScission Leu-Glu-Val-Leu-Phe-Gln��Gly-Pro Amersham-Biosciences GSTrap for GST fusion enzyme TAGZyme  His-tag removal by Exoproteolytic Digestion Qiagen Ni-NTA (6His recomb. enzyme) Intein Site  dithiothreitol cleavage New England Biolabs DTT elimination by dialysis  HRV 3C Protease   Leu-Glu-Val-Leu-Phe-Gln��Gly-Pro Novagen     Ni-NTA (6His recomb. enzyme) SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO  Invitrogen – Life Technologies, LifeSensors Ni-NTA (6His recomb. enzyme)